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- domestic rabbit 单克隆 (J.924.2)
反应物种: American tobacco, 酿酒酵母S288C, 人类, 小鼠, 大鼠, 斑马鱼, 秀丽隐杆线虫
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
Immunoprecipitation of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
规格: 100µL
价格: 美国345
至产商 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 秀丽隐杆线虫
应用: 免疫细胞化学 - 小鼠 单克隆 (APH3-64)
反应物种: 人类, 小鼠, 大鼠, 鸡, 牛, 黑腹果蝇, 秀丽隐杆线虫
修饰: 磷酸化
应用: 免疫印迹, 酶联免疫吸附测定, 免疫沉淀
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基因信息 - 秀丽隐杆线虫 his-43
- 描述:Histone H2A
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- his-43 抗体
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