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- domestic rabbit 多克隆
反应物种: 酿酒酵母S288C
应用: 免疫印迹
Western Blot: Hsp104 Antibody [NBP1-97473] - Analysis of HSP104 (yeast), pAb: Lane 1: MW Marker, Lane 2: Yeast Cell Lysate.
规格: 0.05 mg (also 0.025 mg, 0.2 mg)
价格: 309美元
至产商 - domestic rabbit 多克隆 (Antiserum 8-1)
反应物种: 酿酒酵母S288C
应用: 免疫印迹
Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Heat Shock Protein 104 (PA3-016A) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Heat Shock Protein 104 (PA3-016A) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
规格: 100微升
价格: 美国400.00
至产商
基因信息 - 酿酒酵母S288C HSP104
- 描述:chaperone ATPase HSP104
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- HSP104 抗体
- HSP104 cDNA
- HSP104 蛋白
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