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- 大鼠 单克隆 (YL1/2)
反应物种: African green monkey, 酿酒酵母S288C, Dictyostelium discoideum, 人类, 小鼠, 大鼠, 犬, pigs, fruit fly , 非洲爪蛙, domestic rabbit
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 64 - 大鼠 单克隆 (YOL1/34)
反应物种: 酿酒酵母S288C, 白色念珠菌, 人类, 小鼠, fruit fly
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-冰冻切片
文章摘录数: 18 - 大鼠 单克隆 (YOL1/34)
反应物种: 酿酒酵母S288C, 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片 - domestic rabbit 单克隆 (CI-20)
反应物种: 酿酒酵母S288C
应用: 免疫印迹
Western blot analysis of alpha Tubulin expression in Saccharomyces cerevisiae (yeast) cell lysate (M30932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUB1 monoclonal antibody (Catalog # M30932) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUB1
规格: 100微升
价格: 315美元
至产商
基因信息 - 酿酒酵母S288C TUB1
- 描述:alpha-tubulin TUB1
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产品类型
- TUB1 抗体
- TUB1 cDNA
物种
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