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- 兔 多克隆
反应物种: 酿酒酵母S288c
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
Western blot of APG8 fusion protein. Anti-APG8 antibody generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilution of Rockland's IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX (code # 611-103-122). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel. Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no reactivity of anti-APG8 with other UBLs. This data indicates that anti-APG8 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.
规格: 500微克
价格: 432美元
至产商 - 兔 多克隆
反应物种: 酿酒酵母S288c
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基因信息 - 酿酒酵母S288c ATG8
- 同义词:APG8; AUT7; CVT5; ubiquitin-like protein ATG8
- 描述:ubiquitin-like protein ATG8
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产品类型
- ATG8 抗体
- ATG8 cDNA
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