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- 兔 多克隆
反应物种: 酿酒酵母S288c
应用: 免疫印迹, 酶联免疫吸附测定, 免疫沉淀
Western blot using Rockland's Affinity Purified anti-Yeast CHK1 antibody shows detection of a bands corresponding to CHK1 in Saccharomyces cerevisiae lysates. Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 kDa. Lane 2 shows a predominant band at ~38 kDa. Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ?g of peptide immunogen (lanes 3 and 4 respectively). Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
规格: 100微克
价格: 369美元
至产商 - 兔 多克隆
反应物种: 酿酒酵母S288c
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基因信息 - 酿酒酵母S288c CHK1
- 同义词:serine/threonine protein kinase CHK1
- 描述:serine/threonine protein kinase CHK1
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- CHK1 抗体
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