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- 兔 多克隆
反应物种: fission yeast, 粗糙脉孢菌, 酿酒酵母S288c, 面包酵母
修饰: 磷酸化
应用: 免疫印迹, 酶联免疫吸附测定, 染色质免疫沉淀
文章摘录数: 24 - 兔 多克隆
反应物种: 酿酒酵母S288c, fission yeast
修饰: 磷酸化
应用: 免疫印迹
文章摘录数: 2 - 兔 单克隆 (EPR17588)
反应物种: 酿酒酵母S288c
应用: 免疫印迹, 酶联免疫吸附测定, 染色质免疫沉淀 , dot blot - 兔 单克隆 (DEE-8)
反应物种: 酿酒酵母S288c
应用: 免疫印迹
Western blot analysis of Phospho-Histone H2A (S129) expression in Saccharomyces cerevisiae treated with Methyl methanesulfonate (P30931). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTA2 monoclonal antibody (Catalog # P30931) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTA2
规格: 100 ug/vial
价格: 299美元
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基因信息 - 酿酒酵母S288c HTA2
- 同义词:H2A2; histone H2A
- 描述:histone H2A
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产品类型
- HTA2 抗体
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