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- 兔 单克隆 (DEH-8)
反应物种: 酿酒酵母S288c
应用: 免疫印迹
Western blot analysis of Histone H2B expression in Saccharomyces cerevisiae cell lysate (M30930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTB1 monoclonal antibody (Catalog # M30930) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTB1
规格: 100 ug/vial
价格: 299美元
至产商 - 兔 单克隆 (DEI-8)
反应物种: 酿酒酵母S288c
应用: 免疫印迹
Western blot analysis of Histone H2B expression in Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate (P30930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTB1 monoclonal antibody (Catalog # P30930) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTB1
规格: 100 ug/vial
价格: 299美元
至产商 - 兔 单克隆 (EPR18094)
反应物种: 酿酒酵母S288c
应用: 免疫印迹, 染色质免疫沉淀
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基因信息 - 酿酒酵母S288c HTB1
- 同义词:SPT12; histone H2B
- 描述:histone H2B
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产品类型
- HTB1 抗体
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