Your Filters
产品
- domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 犬
应用: 免疫印迹, 免疫细胞化学, 染色质免疫沉淀
文章摘录数: 1
Figure: WB using PAb to mTOR (human FRB Domain) (Prod. No. ALX-215-065) on total yeast extracts derived from cells expressing FRB-tagged genes coding for three different transcription factors. Separation SDS-PAGE.
Figure 2: Western Blot of mTOR (human FRB Domain), pAb (Prod. No. ALX-215-065) with Hela cells.Method:Two ml of an exponential HeLa suspension culture (2-4x105 cells) was washed twice with PBS quickly without resuspention of the pellet by spinning in a Eppendorf tube and finally with 25% TCA. TCA was carefully removed and pellet respuspended 100µl FSB + ßME (final sample buffer) and the pH adjusted with about a few µl of 1M Tris base (‰ˆ10 µl). The sample was heated at 100°C for 5 min. and sonicated. SDS PAGE was at 7.5%, 20 & 25 µl loaded in lane 1 and 2, respectively. The TCA step is performed to prevent proteolytic breakdown of the very large mTOR.
规格: 1 vial
价格: 434美元
至产商
微博分享
关注我们的微博
- 来邦网
- 英文来邦
基因信息 - 犬 COL4A1
- 描述:collagen type IV alpha 1 chain
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- COL4A1 抗体
外链
问题和意见