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- 小鼠 单克隆 (10E9)
反应物种: 人类, 小鼠, 犬
修饰: 磷酸化
应用: 免疫印迹, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 5 - 小鼠 单克隆 (10E9)
反应物种: 人类, 小鼠, 犬
修饰: 磷酸化
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
Immunofluorescent analysis of Phospho-CREB pSer133 (green) in HeLa cells either left untreated (left panel) or treated with 1uM Camptothecin (right panel) for 20 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-CREB pSer133 monoclonal antibody (Product # MA1-114) at a concentration of 4ug/ml for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight Instrument at 20X magnification.
规格: 100微克
价格: 美国626.00
至产商 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 犬, 鸡, 牛, 斑马鱼
应用: 免疫印迹
基因信息 - 犬 CREB1
- 描述:cAMP responsive element binding protein 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- CREB1 抗体
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