目录: PA5-19489兔 多克隆
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠, 狗, 鸡, 牛, 果蝇
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
目录: PA5-22060兔 多克隆
反应物种: 人类, 小鼠, 大鼠, 果蝇
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
目录: PA5-29444兔 多克隆
反应物种: 人类, 小鼠, 大鼠, 鸡, 果蝇
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
Western Blot analysis using anti-alpha Tubulin 4a Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG. Primary antibody (PA5-29444) diluted at 1:10000
Confocal immunofluorescence analysis of methanol-fixed HeLa using alpha Tubulin 4a (PA5-29444) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (red) at 1:2500.
Immunohistochemical analysis of paraffin-embedded U373 xenograft using alpha Tubulin 4a (PA5-29444) antibody at 1:500 dilution.
基因信息 - 果蝇 alphaTub84B
- 同义词:ALPHA 84C; Alpha-Tubulin84B; BEST:LD32507; CG1913; DM1alpha; DTA1; Dmel\CG1913; TBA1_DROME; Talpha1; Tub; Tub 84B; Tub1C; Tuba84B; Tubalpha1; a-tub84B; a1t; aTub84B; alpha-Tub; alpha-Tub84B; alpha-tub; alpha-tub84B; alpha-tubulin; alpha-tubulin 84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphaTUB; alphaTUB84B; alphaTub; alphaTub1; alphaTub84; alphat-1; alphatub; alphatub84B; anon-EST:Liang-1.59; anon-EST:Liang-2.30; chr3R:2914276..2914416; clone 1.59; clone 2.30; l(3)84Bd; l(3)g3; ms(3)nc33; nc33; tub; tubA84B; tubalpha1; tubulin; alpha-Tubulin at 84B; CG1913-PA; Tubulin-alpha1; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; alpha-Tubulin 84B; alpha-tubulin 1; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alphaT; alphaTub84B-PA; alphaTubulin 84B; alphaTubulin84B; alpha[] tubulin; alpha[]-tubulin; alphatubulin; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha1; tubulin alpha1 promoter; tubulin(alpha1); tubulin1alpha; tubulin84B
- 描述:alpha-Tubulin at 84B
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- alphaTub84B 抗体
- alphaTub84B cDNA