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- domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
Figure 1. Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat heart tissue lysates,. Lane 3: rat kidney tissue lysates,. Lane 4: rat ovary tissue lysates,. Lane 5: mouse brain tissue lysates,. Lane 6: mouse heart tissue lysates,. Lane 7: mouse kidney tissue lysates,. Lane 8: mouse testis tissue lysates,. Lane 9: mouse SP20 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KBTBD2 antigen affinity purified polyclonal antibody (Catalog # A15277-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KBTBD2 at approximately 71KD. The expected band size for KBTBD2 is at 71KD.
Figure 2. Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates,. Lane 3: human K562 whole cell lysates,. Lane 4: human U2OS whole cell lysates,. Lane 5: human THP-1 whole cell lysates,. Lane 6: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KBTBD2 antigen affinity purified polyclonal antibody (Catalog # A15277-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KBTBD2 at approximately 71KD. The expected band size for KBTBD2 is at 71KD.
Figure 3. IF analysis of KBTBD2 using anti- KBTBD2 antibody (A15277-1) . KBTBD2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- KBTBD2 Antibody (A15277-1) overnight at 4°C. DyLight?488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100µg/vial
价格: 315美元
至产商 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
基因信息 - 小鼠 Kbtbd2
- 同义词:BC022962; Bklhd1; mKIAA1489
- 描述:kelch repeat and BTB (POZ) domain containing 2
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- Kbtbd2 抗体
- Kbtbd2 基因敲低
- Kbtbd2 cDNA
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