Your Filters
产品
- domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Figure 1. Western blot analysis of BUB1 using anti-BUB1 antibody (PB9135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human BUB1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody (Catalog # PB9135) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 39KD. The expected band size for BUB1 is at 39KD.
Figure 2. Western blot analysis of BUB1 using anti- BUB1 antibody (PB9135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates,. Lane 2: human Hela whole cell lysates,. Lane 3: human 293T whole cell lysates, . Lane 4: human A431 whole cell lysates,. Lane 5: human Caco-2 whole cell lysates,. Lane 6: human PC-3 whole cell lysates,. Lane 7: human MCF-7 whole cell lysates,. Lane 8: human U2OS whole cell lysates,. Lane 9: rat testis tissue lysates,. Lane 10: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- BUB1 antigen affinity purified polyclonal antibody (Catalog # PB9135) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130KD. The expected band size for BUB1 is at 122KD.
规格: 100μg/vial
价格: 315美元
至产商 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片 - domestic rabbit 重组 (JE64-70)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪 - domestic rabbit 单克隆 (EPR12369)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪
基因信息 - 大鼠 Aamp
- 描述:angio-associated, migratory cell protein
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- Aamp 抗体
外链
问题和意见