大鼠 Hspb1 抗体 | 15 (1 knockout-validated) products in Validated Antibody Database; 24 cited in the literature; 76 total from 13 suppliers

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HSP27单克隆抗体(G3.1)
赛默飞世尔
目录: MA3-015

产品总数: 19

小鼠单克隆 (G3.1)
反应物种: 人类, 小鼠, 大鼠, 犬
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 35

Western blot analysis of Heat Shock Protein 27 (HSP27) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP27 monoclonal antibody (Product # MA3-015) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
规格: 100微升
价格: 美国477.00
至产商
磷酸化HSP27(Ser15)多克隆抗体
赛默飞世尔
目录: PA1-016

产品总数: 19

domestic rabbit 多克隆
反应物种: 人类, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunoprecipitation of Phospho Heat Shock Protein (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). HeLa cell lysate (50ug) was loaded as a positive control (left lane). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight rotating at 4°C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho-HSP27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
规格: 100微克
价格: 美国400.00
至产商
HSP27多克隆抗体
赛默飞世尔
目录: PA1-017

产品总数: 19

domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 2

规格: 100微克
价格: 美国471.00
至产商
抗Hsp27抗体[G3.1]
艾博抗（上海）贸易有限公司
目录: ab2790

产品总数: 11

小鼠单克隆 (G3.1)
反应物种: 人类, 小鼠, 大鼠, 犬, 鸡, pigs
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 14

规格: 100微克
价格:
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抗Hsp27抗体[8A7]
艾博抗（上海）贸易有限公司
目录: ab79868

产品总数: 11

小鼠单克隆 (8A7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 4

规格: 100微克
价格:
至产商
抗Hsp27抗体[5D7]
艾博抗（上海）贸易有限公司
目录: ab114067

产品总数: 11

小鼠单克隆 (5D7)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 4

规格: 100微升
价格:
至产商
HSP 27(F-4)抗体
圣克鲁斯生物技术
目录: sc-13132

产品总数: 8

小鼠单克隆 (F-4)
反应物种: 人类, 小鼠, 大鼠, 牛
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫印迹基因敲除验证
文章摘录数: 25

规格: 200微克/毫升
价格:
至产商
HSP 27(HSP25-19)抗体
圣克鲁斯生物技术
目录: sc-51956

产品总数: 8

小鼠单克隆 (HSP25-19)
反应物种: 人类, 小鼠, 大鼠, 鸡
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀
文章摘录数: 5

规格: 50微克/0.5毫升
价格:
至产商
p-HSP 27(5B9)抗体
圣克鲁斯生物技术
目录: sc-81498

产品总数: 8

小鼠单克隆 (5B9)
反应物种: 人类, 小鼠, 大鼠, 犬
修饰: 磷酸化
应用: 免疫印迹, 免疫沉淀
文章摘录数: 1

规格: 50微克/0.5毫升
价格:
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HSP27抗体
Novus Biologicals
目录: NB120-5579

产品总数: 16

domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 3

规格: 100 ug (also 0.025 mg)
价格: 399美元
至产商
HSP27抗体(G3.1)
Novus Biologicals
目录: NBP2-32972-0.1mg

产品总数: 16

小鼠单克隆 (G3.1)
反应物种: 人类, 小鼠, 大鼠, 鸡, 黑猩猩
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 2

Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).
规格: 0.1 mg (also 0.02 mg)
价格: 419美元
至产商
HSP27抗体(6H11)
Novus Biologicals
目录: NBP2-25149

产品总数: 16

小鼠单克隆 (6H11)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
文章摘录数: 1

规格: 0.1 ml (also 0.025 ml)
价格: 399美元
至产商
HSP25/HSP27抗体
StressMarq Biosciences
目录: SMC-114

小鼠单克隆 (8A7)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
文章摘录数: 2

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (SMC-114). Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (SMC-114) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation. Courtesy of: Mike Welsh, Umich.
规格: 100微克
价格:
至产商
磷酸化HSP27(S78/S82)抗体
安迪生物R&D
目录: AF2314

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学
文章摘录数: 1

Detection of Human and Mouse Phospho-HSP27 (S78/S82) antibody by Western Blot.
规格: 50 ug (also 25 ug)
价格: 389美元
至产商
HSP27抗体
安迪生物R&D
目录: AF15801

domestic goat 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Detection of Human and Mouse HSP27 antibody by Western Blot.
规格: 50 ug (also 25 ug)
价格: 429美元
至产商
兔抗人类加热休克27kDa蛋白1多克隆抗体
MyBioSource
目录: MBS716588

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 酶免疫法

规格: 0.05毫升
价格:
至产商
Anti-Hsp27/Hspb1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00676

产品总数: 5

domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Figure 1. Western blot analysis of Hsp27 using anti-Hsp27 antibody (A00676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates,. Lane 2: rat lung tissue lysates,. Lane 3: mouse spleen tissue lysates,. Lane 4: mouse skeletal muscle tissue lysates,. Lane 5: mouse lung tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp27 antigen affinity purified polyclonal antibody (Catalog # A00676) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp27 at approximately 27KD. The expected band size for Hsp27 is at 27KD.

Figure 2. IHC analysis of Hsp27 using anti-Hsp27 antibody (A00676). Hsp27 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hsp27 Antibody (A00676) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Hsp27 using anti-Hsp27 antibody (A00676). Hsp27 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hsp27 Antibody (A00676) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
抗HSP27 Hspb1单克隆抗体
武汉博士德生物工程有限公司
目录: MA1048

产品总数: 5

小鼠单克隆 (IAP-9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Boster Kit Box
规格: 100μg/vial
价格: 315美元
至产商
抗磷酸化Hsp27(S82)HSPB1兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00676-1

产品总数: 5

domestic rabbit 单克隆 (FGD-8)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀

Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock (P00676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
规格: 100微升
价格: 315美元
至产商
HSPB1单克隆抗体, 克隆8A7
亚诺法生技股份有限公司
目录: MAB2184

产品总数: 6

小鼠单克隆 (8A7)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛
应用: 免疫印迹, 免疫细胞化学

规格: 100微克
价格:
至产商
HSPB1单克隆抗体, 克隆5D7
亚诺法生技股份有限公司
目录: MAB10690

产品总数: 6

小鼠单克隆 (5D7)
反应物种: 人类, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

规格: 100微升
价格:
至产商
HSPB1(磷酸化S15)多克隆抗体
亚诺法生技股份有限公司
目录: PAB24730

产品总数: 6

domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹

规格: 100微升
价格:
至产商
Hsp25/Hsp27抗体
Rockland Immunochemicals
目录: 200-301-F55

小鼠单克隆 (8A7)
反应物种: 小鼠, 大鼠, 犬, 牛
应用: 免疫印迹, 免疫组化, 免疫沉淀

Western Blot of mouse anti-Hsp25/Hsp27 antibody. Lane 1: molecular weight markers. Lane 2: rat lung tissue lysates. Load: 35 µg per lane. Primary antibody: Hsp25/Hsp27 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 20.2 kDa/ ~24kDa for Hsp25/Hsp27. Other band(s): none.
规格: 100微克
价格:
至产商
抗Hsp25/Hsp27抗体
QED Bioscience
目录: 11110

小鼠单克隆 (8A7)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
规格: 100微克
价格: 388美元
至产商
Hsp27(HSPB1, 热休克蛋白乙1, HspB1, 加热休克27kD蛋白, HSP 27)
US Biological
目录: H1830-59

domestic rabbit 多克隆
反应物种: 大鼠

规格: 100微克
价格:
至产商
磷酸化HSP27(Ser82)(D1H2)XP®兔单克隆抗体
赛信通（上海）生物试剂有限公司
目录: 9709

产品总数: 4

domestic rabbit 单克隆 (D1H2)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛
修饰: 磷酸化
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, reverse phase protein lysate microarray
文章摘录数: 14

规格:
价格:
至产商