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- domestic rabbit 单克隆 (EPR18012-257)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 1 - domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Figure 1. Western blot analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: mouse Neuro-2a whole cell lysates,. Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody (Catalog # A01373-2) at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.
Figure 2. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2). IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2). IGFBP2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商 - domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹
Anti- IGFBP2 Picoband antibody, PB9605, Western blotting. All lanes: Anti IGFBP2 (PB9605) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Liver Tissue Lysate at 50ug. Lane 3: Human Placenta Tissue Lysate at 50ug. Predicted bind size: 35KD. Observed bind size: 35KD
规格: 100μg/vial
价格: 315美元
至产商 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
基因信息 - 大鼠 Igfbp2
- 同义词:BRL-BP; IBP-2; IGFBP-2; ILGFBPA
- 描述:insulin-like growth factor binding protein 2
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- Igfbp2 抗体
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