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- domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
Figure 1. Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (A04548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates,. Lane 2: mouse kidney tissue lysates,. Lane 3: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # A04548) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 17KD. The expected band size for Lymphotactin is at 12KD.
规格: 100µg/vial
价格: 315美元
至产商 - domestic rabbit 多克隆
反应物种: 大鼠
应用: 免疫印迹, 免疫沉淀 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹 - domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹 - Lymphotactin (C Motif Chemokine 1, Cytokine SCM-1, Small-inducible Cytokine C1, ...
US Biological
目录: 029835domestic rabbit 多克隆
反应物种: 大鼠
基因信息 - 大鼠 Xcl1
- 同义词:Ltn; Scyc1
- 描述:X-C motif chemokine ligand 1
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产品类型
- Xcl1 抗体
- Xcl1 蛋白
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