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- 小鼠 单克隆 (4D2)
反应物种: 人类, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片
文章摘录数: 5
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.
规格: 100 ug (also 25 ug)
价格: 449美元
至产商 - domestic rabbit 单克隆 (Rho 1D4)
反应物种: 人类, 小鼠, 大鼠, 牛, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 放射免疫分析法
Western Blot: Rhodopsin Antibody (Rho 1D4) [NBP2-81059] - Western Blot using anti-Rhodopsin antibody (Rho 1D4) [NBP2-81059]. Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 [NBP2-81059] at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat ( Rattus norvegicus ) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). NBP2-81059 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.
规格: 0.2 mg (also 0.025 mg)
价格: 469美元
至产商 - 小鼠 单克隆 (Rho 1D4)
反应物种: 人类, 小鼠, 大鼠, 牛, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 放射免疫分析法
ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.
规格: 0.2 mg (also 0.025 mg)
价格: 469美元
至产商 - 小鼠 重组 (Rho 1D4)
反应物种: 人类, 小鼠, 大鼠, 牛, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀 - domestic rabbit 重组 (Rho 1D4)
反应物种: 人类, 小鼠, 大鼠, 牛, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
基因信息 - 斑马鱼 exorh
- 同义词:Ex; ExR; exo-rod
- 描述:extra-ocular rhodopsin
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