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- domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 酶联免疫吸附测定
Figure 1. Western blot analysis of MED14 using anti-MED14 antibody (A04799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,. Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED14 antigen affinity purified polyclonal antibody (Catalog # A04799-2) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED14 at approximately 160KD. The expected band size for MED14 is at 160KD.
规格: 100 ug/vial
价格: 315美元
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基因信息 - 斑马鱼 med14
- 同义词:EXLM1; crsp150; crsp2; trap170; wu:fd21c05; wu:fk28h11
- 描述:mediator complex subunit 14
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- med14 抗体
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