产品简要
公司名称 :
ACROBiosystems
产品类型 :
蛋白
产品名称 :
Human Akt1 Protein, His,Strep II Tag
目录 :
AK1-H5283
规格 :
25微克, 500微克
价格 :
350美元, 1600美元
更多信息或购买 :
产品信息
公司名称 :
ACROBiosystems
产品类型 :
蛋白
目录 :
AK1-H5283
产品名称 :
Human Akt1 Protein, His,Strep II Tag
规格 :
25微克, 500微克
价格 :
350美元, 1600美元
规格&价格 :
$350/25微克, $1600/500微克
靶标 :
Akt1
宿主物种 :
人类
By Tag :
His Tag & Strep Tag
研究 :
For Research Use Only
来源 :
Human Akt1, His,Strep II Tag (AK1-H5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 480 (Accession # AAH00479).
内毒素 :
Less than 1.0 EU per μg by the LAL method.
纯度 :
>92% as determined by reduced SDS-PAGE.
SDS-PAGE Remark(R&NR) :
Human Akt1, His,Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
配方 :
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
重组 :
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
储存 :
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: ● -20°C to -70°C for 12 months in lyophilized state; ● -70℃ for 3 months under sterile conditions after reconstitution.
背景 :
RAC-alpha serine/threonine-protein kinase (AKT1) is also known PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt and RAC-PK-alpha, which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily and is expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase).
参考文献 :
(1) Jones P.F., et al., 1991, Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175. (2) Goshima N., et al., 2008 Nat. Methods 5:1011-1017. (3) Delcommenne M., et al ., 1998, Proc. Natl. Acad. Sci. U.S.A. 95:11211-11216.
更多信息或购买 :
公司信息
ACROBiosystems
1 Innovation Way Newark, DE 19711, USA
order@acrobiosystems.com
http://www.acrobiosystems.com
1-800-810-0816
ACROBiosystems is a leading manufacturer of recombinant proteins and other critical reagents to support the development of target therapeutics, vaccines and diagnostic kits.

Our team has worked tirelessly since the genomic sequence of SARS-CoV-2 was first published, developing a serial of critical reagents to fight COVID-19. Our team has successfully developed a new neutralizing antibody titer serologic assay kit for COVID-19 vaccine evaluation (Cat.No.TAS-K022) which really showed excellent performance.

As the emerging variants may have impacted on the development of the COVID-19 epidemic, our team is also going full steam ahead on developing a collection of spike mutants for the emerging variants of SARS-COV-2. The reagents can be used to evaluate the efficacy of the antibodies and vaccination.

We have also developed an extensive collection of recombinant proteins to support the development for bispecific antibodies, antibody drug conjugates and cell therapies.