产品简要
公司名称 :
Abbexa
产品类型 :
ELISA/assay
产品名称 :
Human Monocyte Chemotactic Protein 1 (CCL2) ELISA Kit
目录 :
abx050025
规格 :
96试验
价格 :
652.5美元
更多信息或购买 :
产品信息
目录代码 :
abx050025
靶标 :
Monocyte Chemotactic Protein 1
产品名称 :
Human Monocyte Chemotactic Protein 1 (CCL2) ELISA Kit
简称 :
CCL2
Long Name :
Monocyte Chemotactic Protein 1
产品类型 :
酶联免疫吸附试剂盒
规格1 :
96试验
标价1(美元) :
652.5美元
产品描述 :
Human Monocyte Chemotactic Protein 1 ELISA Kit is an ELISA Kit against Monocyte Chemotactic Protein 1.
交叉活性 :
人类
别名 :
CCL2, C-C Motif Chemokine Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE, Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je), Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2, Monocyte Chemoattractant Protein-1, Monocyte Chemotactic Protein 1, Chemokine (C-C Motif) Ligand 2, Small-Inducible Cytokine A2, SCYA2, MCP-1, HC11, MCAF, MCP1, Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je, Monocyte Chemoattractant Protein 1, C-C Motif Chemokine 2, HSMCR30, GDCF-2, SMC-CF
应用摘要 :
酶联免疫吸附测定
储存 :
Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
UNSPSC编码 :
41116126
试验范围 :
62.5 pg/ml - 4000 pg/ml
敏感性 :
37.5 pg/ml
ELISA Detection :
Colorimetric
酶联免疫吸附测定类型 :
Sandwich
酶联免疫吸附测定数据 :
定量
样品类型 :
Serum, plasma and other biological fluids.
Assay Principle :
This kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient CCL2 will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow colour is proportional to the CCL2 amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of CCL2 can be calculated.
Kit Components :
Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer
Material Required But Not Provided :
37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker
Sample Collection/Preparation :
Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4°C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20°C or -80°C. Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 × g within 30 minutes of collection. Assay immediately or aliquot and store at -20°C or -80°C. Avoid hemolysis and high cholesterol samples. Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type – this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 × g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20°C.
Reagent Preparation :
1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol. 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol. 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure :
1) Set standard, test samples and control wells. 2) Aliquot 100 µl of diluted standard into the standard wells. 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well. 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 90 mins at 37 °C. 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C. 6) Wash 3 times. 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C. 8) Wash 5 times. 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C. 10) Aliquot 50 µl of Stop Solution. 11) Measure the OD at 450 nm.
实验方法 :
Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test. 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate. 2. Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C. 3. Remove the cover and discard the liquid. 4. Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C. 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer. 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min. 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step. 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min. 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Results Calculation :
For calculation, average the O.D.450 duplicate readings for each reference standard and each sample and substract the average control (zero) O.D.450 reading. The standard curve can be plotted as the relative O.D.450 of each reference standard solution (Y) vs. the respective concentration of each standard solution (X). The CCL2 concentration of the samples can be interpolated from the standard curve.
Assay Precision :
Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Monocyte Chemotactic Protein 1 (CCL2) were were tested 20 times on one plate, respectively. . Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Monocyte Chemotactic Protein 1 (CCL2) were tested on 3 different plates, 8 replicates in each plate. . CV (%) = (Standard Deviation / mean) × 100 . Intra-Assay: CV . Inter-Assay: CV
Availability :
Shipped within 5-12 working days.
提示 :
This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
更多信息或购买 :
公司信息
Abbexa
Abbexa LTD, Cambridge, UK
Abbexa LLC, Houston, USA
info@abbexa.com
https://www.abbexa.com
44 (0)1223 75595
1 832 327 7413
公司总部: 英国
Abbexa is a biotechnology company based in Cambridge, UK, specializing in high quality, reliable research reagents for scientific research. We work in partnership with academic institutions, hospitals and laboratories across the world to bring an integrated source of antibodies, proteins, ELISA kits and other biological reagents. We are dedicated to providing excellent customer service, quality and affordable products to enable scientists to do better science. For more information, please visit http://www.abbexa.com/.