ELISA/assay

人类50% 补体溶血(50%补体溶血单位)酶联免疫吸附试剂盒

abx051071

96试验

572.75美元

50% 补体溶血(50%补体溶血单位)

人类50% 补体溶血(50%补体溶血单位)酶联免疫吸附试剂盒

50%补体溶血单位

50% 补体溶血

酶联免疫吸附试剂盒

96试验

572.75美元

Human 50% Complement Hemolysis ELISA Kit is an ELISA kit against Human 50% Complement Hemolysis.

人类

50% 补体溶血, 50%补体溶血单位

酶联免疫吸附测定

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

41116126

2 U/毫升-150 U/毫升

0.6 U/毫升

定量

Serum, plasma, cell culture supernatants and tissue homogenates.

Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer

37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker

Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4°C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20°C or -80°C. Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 × g within 30 minutes of collection. Assay immediately or aliquot and store at -20°C or -80°C. Avoid hemolysis and high cholesterol samples. Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type – this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 × g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20°C.

1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol. 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol. 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

Shipped within 5-10 working days.

This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.