产品简要
公司名称 :
Abbexa
产品类型 :
ELISA/assay
产品名称 :
人类表皮生长因子(EGF)酶联免疫吸附试剂盒
目录 :
abx151381
规格 :
96试验
价格 :
609美元
更多信息或购买 :
产品信息
目录代码 :
abx151381
靶标 :
EGF
产品名称 :
人类表皮生长因子(EGF)酶联免疫吸附试剂盒
简称 :
EGF
产品类型 :
酶联免疫吸附试剂盒
规格1 :
96试验
规格2 :
5 × 96 tests
规格3 :
10 × 96 tests
价格1(美元) :
609美元
价格2(美元) :
2,900.00
价格3(美元) :
5,481.00
产品描述 :
Human Epidermal Growth Factor (EGF) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human EGF concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF) No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.
UniProt Primary AC :
P01133
UniProt数据库进入名 :
EGF_HUMAN
KEGG :
hsa:1950
String :
9606.ENSP00000265171
交叉活性 :
人类
检测过的应用 :
酶联免疫吸附测定
储存 :
Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
UNSPSC编码 :
41116126
试验范围 :
3.9 pg/ml - 250 pg/ml
敏感性 :
< 1.5 pg/ml
检测方法 :
Colorimetric
试验类型 :
Sandwich
Assay Data :
定量
样品类型 :
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Kit Components :
The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer
Material Required But Not Provided :
37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker
实验方法 :
This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test. 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate. 2. Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C. 3. Remove the cover and discard the liquid. 4. Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C. 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer. 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min. 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step. 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min. 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Availability :
Shipped within 5-7 working days.
提示 :
This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
干冰 :
No
更多信息或购买 :
公司信息
Abbexa
Abbexa LTD, Cambridge, UK
Abbexa LLC, Houston, USA
info@abbexa.com
https://www.abbexa.com
44 (0)1223 75595
1 832 327 7413
公司总部: 英国
Abbexa is a biotechnology company based in Cambridge, UK, specializing in high quality, reliable research reagents for scientific research. We work in partnership with academic institutions, hospitals and laboratories across the world to bring an integrated source of antibodies, proteins, ELISA kits and other biological reagents. We are dedicated to providing excellent customer service, quality and affordable products to enable scientists to do better science. For more information, please visit http://www.abbexa.com/.