ELISA/assay

人类细胞间粘附分子1(细胞间粘附分子 1)酶联免疫吸附试剂盒

abx351184

96试验

543.75美元

细胞间粘附分子1(细胞间粘附分子 1)

人类细胞间粘附分子1(细胞间粘附分子 1)酶联免疫吸附试剂盒

细胞间粘附分子 1

酶联免疫吸附试剂盒

96试验

543.75美元

Human Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Intercellular Adhesion Molecule 1 (ICAM1) concentrations in tissue homogenates, cell lysates and other biological fluids.

P05362

ICAM1_HUMAN

147840

5344

hsa:3383

9606.ENSP00000264832

人类

酶联免疫吸附测定

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

41116126

0.312 ng/ml - 20 ng/ml

0.19 ng/ml

Colorimetric

Sandwich

定量

Tissue homogenates, cell lysates and other biological fluids.

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer

37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test. 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate. 2. Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C. 3. Remove the cover and discard the liquid. 4. Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C. 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer. 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min. 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step. 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min. 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.

Shipped within 5-12 working days.

This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

No