ELISA/assay

Human 26S Proteasome Non-ATPase Regulatory Subunit 4 (PSMD4) ELISA Kit

abx572053

96试验

797.5美元

26S Proteasome Non-ATPase Regulatory Subunit 4 (PSMD4)

Human 26S Proteasome Non-ATPase Regulatory Subunit 4 (PSMD4) ELISA Kit

PSMD4

酶联免疫吸附试剂盒

96试验

5 × 96 tests

10 × 96 tests

797.5美元

3,799.00

7,177.50

Human 26S Proteasome Non-ATPase Regulatory Subunit 4 (PSMD4) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human 26S Proteasome Non-ATPase Regulatory Subunit 4 (PSMD4) concentrations in tissue homogenates, cell lysates and other biological fluids. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

P55036

PSMD4_HUMAN

PSMD4

601648

NP_002801.1, NM_002810.2

9561

hsa:5710

ENSG00000159352

9606.ENSP00000357879

人类

酶联免疫吸附测定

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

41116126

78 pg/ml - 5000 pg/ml

< 31 pg/ml

Colorimetric

Sandwich

定量

Tissue homogenates, cell lysates and other biological fluids.

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer

37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test. 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate. 2. Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C. 3. Remove the cover and discard the liquid. 4. Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C. 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer. 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min. 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step. 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min. 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.

Shipped within 5-7 working days.

This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

No