抗体

Monoclonal Antibody to Caspase-8 (Clone: ABM14C1)

10-1019

100微克

260美元

单克隆

小鼠

nonconjugated

ABM14C1

人类, 小鼠

免疫印迹, 免疫组化, 流式细胞仪

Monoclonal Antibody to Caspase-8 (Clone: ABM14C1)

抗体

小鼠

单克隆

未共轭

ABM14C1

小鼠IgG1

100微克

260美元

104.238.111.116/Abeomics/abeo_images_2/10-1019/4.jpg

Fig-4: Immunohistochemical analysis of Caspase-8 in human Esophagus using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 µg/ml.

104.238.111.116/Abeomics/abeo_images_2/10-1019/5.jpg

Fig-5: Immunohistochemical analysis of Caspase-8 in human Tonsil using anti-Caspase-8 antibody (Clone: ABM14C1) at 5 µg/ml.

104.238.111.116/Abeomics/abeo_images_2/10-1019/6.jpg

Fig-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 4 µg/ml on EL-4 lysate.

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.The Active/Cleaved Caspase-8 polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-8. Whereas the antisera has a strong preference for active/cleaved caspase-8, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-8 as well as intermediate caspase-8 cleavage fragments.

小鼠, 人类

M, H

Apoptotic cysteine protease, Apoptotic protease Mch-5, FADD-homologous ICE/ced-3-like protease, CE-like apoptotic protease 5

A partial length recombinant protein (a.a 179-385) of Caspase-8 was used as the immunogen for this antibody.

蛋白质G色谱

PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

叠氮化钠

0.5毫克/毫升

Western Blot Analysis 4-6 µg/ml, FACS: 0.5-1 µg/10^6, IHC: 5-10 µg/ml. Pack of 25 µg is also availabe .

免疫组化, 流式细胞仪, 免疫印迹

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.