抗体

Monoclonal Antibody to RIG-I (Clone: ABM4H29)

10-4121

100微克

240美元

单克隆

小鼠

nonconjugated

ABM4H29

免疫印迹, 免疫组化, 流式细胞仪

Monoclonal Antibody to RIG-I (Clone: ABM4H29)

抗体

小鼠

单克隆

未共轭

ABM4H29

小鼠IgG1

Kappa

100微克

240美元

104.238.111.116/Abeomics/abeo_images_2/10-4121/4.jpg

Fig-4: Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 µg/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (10-4121 Abeomics). Goat anti-mouse PE conjugate was used as secondary.

RIG-I (retinoic-acid-inducible gene I), also known as DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58) is a 925-residue cytoplasmic viral RNA receptor, critically involved in the activation of the innate immune response to RNA virus infection. It is a member of the RIG-I-like receptor (RLR) family and is an essential intracellular sensor for several 5-triphosphorylated RNA viruses. RIG-I elicits its antiviral interferon (IFN) responses by recognizing viral double-stranded RNAs (dsRNAs). Structurally it comprises a helicase domain, a C-terminal domain, and N-terminal CARDs (caspase activation recruitment domains) involved in activating MAVS (mitochondrial antiviral signaling protein). Upon binding of 5'-triphosphorylated RNA, RIG-I undergoes conformational changes and post-translational modifications that allow multimerization and interaction with the mitochondrial antiviral signaling protein (MAVS).

人类

H

DDX58

A partial length recombinant human RIG-I protein (amino acids 1-220) was used as the immunogen for this antibody.

蛋白质G色谱

PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

叠氮化钠

0.5毫克/毫升

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 15 µg/ml, Flowcytometric analysis: 0.5-1µg/10^6 cells. Pack of 25 µg is also availabe .

免疫组化, 流式细胞仪, 免疫印迹

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.