其他

Hela (human cervical epithelioid carcinoma) whole cell lysate (denatured)

L013V1

200微克

Hela (human cervical epithelioid carcinoma) whole cell lysate (denatured)

Whole cell lysate (denatured)

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

子宫颈

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95 C for 5 min, and cooled rapidly.

The lysate is ready to load on SDS-PAGE for Western blotting.

In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).

存放在-80 C。 分成小份儿避免反复冻融

12.5% SDS-PAGE Stained with Coomassie Blue.

Lysate

人类

人类

免疫印迹

200微克

2006-10-10

2014-11-18 13:56:05