其他

HeLa (human cervical epithelioid carcinoma) whole cell lysate (non-denatured)

L013V2

200微克

HeLa (human cervical epithelioid carcinoma) whole cell lysate (non-denatured)

Whole cell lysate (non-denatured)

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

子宫颈

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

In modified RIPA Lysis Buffer.

存放在-80 C。 分成小份儿避免反复冻融

12.5% SDS-PAGE Stained with Coomassie Blue.

Lysate

人类

人类

免疫沉淀, 免疫印迹

200微克

11/30/06

11/18/14 13:56