Geneka Biotechnology

抗体

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

39769

100微升

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片, dot blot, 其他

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

兔

多克隆

Methylated

100微升

血清

Human, Mouse, Other (Wide Range)

Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 ul per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010);the purified IgG version (39791) is likely to work in IF as well.DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.

This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

DB, IF, 免疫组化, 免疫沉淀, MeDIP

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

References: This antibody was used as described below: DB: Ito S. et al. (2010) Nature 466: 1129-1133, Szulwach KE et al. (2011) Nature Neurosci doi:10.1038/nn.2959; Nestor CE. et al. (2011) Genome Res ePub. IHC: MM-CM-<nzel M. et al. (2010) Angew Chem Int Ed Engl 49: 5375 - 5377; Haffner MC. et al. (2011) Oncotarget 2: 627-637, Szulwach KE et al. (2011) Nature Neurosci doi:10.1038/nn.2959. IF: Ito S. et al. (2010) Nature 466: 1129-1133. MeDIP: Nestor CE. et al. (2011) Genome Res ePub. MeDIP-Seq: Stroud et al. (2011) Genome Biology 12:R54.