Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.
This Ago4 antibody was raised against a recombinant protein corresponding to amino acids 1-164 of human Ago4.
Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
References: This antibody has been used as described in the following references:IP: Azuma AM et al. (2010) PNAS 105: 7964-7969 WB: Azuma AM et al. (2010) PNAS 105: 7964-7969