产品简要
公司名称 :
Active Motif
其他品牌 :
Geneka Biotechnology
产品类型 :
抗体
产品名称 :
RNA pol II CTD phospho Ser2 antibody (mAb)
目录 :
61083
规格 :
100微克
价格 :
350美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
3.0e+10
反应物种 :
fission yeast, 人类, 小鼠, S. cerevisiae
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀 , 免疫组化-石蜡切片, ChIP-Seq
更多信息或购买 :
Active Motif 产品网页
文章摘录数: 8
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 人类
  • 免疫组化; 人类
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed 出版商
  • 免疫印迹; 人类; 图 1d
Muhar M, Ebert A, Neumann T, Umkehrer C, Jude J, Wieshofer C, et al. SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis. Science. 2018;360:800-805 pubmed 出版商
  • 免疫印迹; 人类; 图 6a
Winter G, Mayer A, Buckley D, Erb M, Roderick J, Vittori S, et al. BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment. Mol Cell. 2017;67:5-18.e19 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 5h
  • 免疫印迹; 小鼠; 1:1000; 图 3e
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto A, Yang Y, Ntziachristos P, et al. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nat Cell Biol. 2016;18:1127-1138 pubmed 出版商
  • 免疫沉淀; S. cerevisiae; 图 S1
Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed 出版商
  • 免疫印迹; 小鼠; 图 4
Chung H, Xu C, Fuchs A, Mund A, Lange M, Staege H, et al. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3. elife. 2016;5: pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 4b
Garcia Cuellar M, Büttner C, Bartenhagen C, Dugas M, Slany R. Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes. Cell Rep. 2016;15:310-22 pubmed 出版商
  • 染色质免疫沉淀 ; fission yeast; 图 8
Catania S, Pidoux A, Allshire R. Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin. PLoS Genet. 2015;11:e1004986 pubmed 出版商
产品信息
目录代码 :
61083
产品名称 :
RNA pol II CTD phospho Ser2 antibody (mAb)
宿主物种 :
大鼠
克隆性 :
单克隆
抗原修饰 :
磷酸化
规格 :
100微克
标价 :
350美元
克隆 :
3.0e+10
抗体亚型 :
IgG1
交叉活性 :
人类
背景 :
RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.
免疫原 :
This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.
用途 :
ChIP, ChIP-chip, ChIP-Seq, IF, IP, WB
储存 :
Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
产品引用 :
References: This antibody has been used in the following references:ChIP: Chapman RD et al. (2007) Science 318: 1780-1782.IF: Chapman RD et al. (2007) Science 318: 1780-1782.IP: Chapman RD et al. (2007) Science 318: 1780-1782.WB: Chapman RD et al. (2007) Science 318: 1780-1782.
更多信息或购买 :
Active Motif 产品网页
公司信息
Active Motif
787 Kangqiao Road，Building 10, Suite 202，Pudong District，Shanghai, China
techchina@activemotif.com
http://www.activemotif.com
86-21-20926090 400-018-8123
公司总部: 美国