免疫印迹, 免疫组化, 免疫细胞化学
TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelialM-bM-^@M-^Smesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.
This TWIST antibody was raised against a recombinant protein corresponding to full-length mouse TWIST.
IF, 免疫组化, 免疫印迹
Antibodies in solution can be stored at -80M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
References: This antibody has been used in the following references: IF: Kida Y et al. (2007) J Histochem Cytochem 55: 661-673.IHC: Gitelman I et al. (1997) Dev Biol 189: 205-214; Eckert MA et al. (2011) Cancer Cell 19: 372-386; Kida Y et al. (2007) J Histochem Cytochem 55: 661-673.WB: Gitelman I et al. (1997) Dev Biol 189: 205-214; Casas et al. (2011) Cancer Res 71: 245-254.