Geneka Biotechnology

抗体

Histone H4ac (pan-acetyl) antibody (mAb)

61337

100微克

350美元

单克隆

小鼠

未共轭

3HH4-3H10

免疫印迹, 染色质免疫沉淀

Histone H4ac (pan-acetyl) antibody (mAb)

小鼠

单克隆

乙酰化

100微克

350美元

3HH4-3H10

IgG1k

Human, Other (Wide Range)

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

This antibody was raised against a peptide corresponding to amino acid residue(s) 1- 24 of human histone H4 acetylated at lysines 5, 8, 12 and 16. Due to sequence similarity, cross-reactivity has been observed with acetylated Histone H2B.

ChIP, 免疫印迹

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

References: The use of this antibody is described in the following reference:ChIP: Dilworth, F.J. et al. (2000) Mol Cell 6:1049-1058.