产品简要
公司名称 :
Active Motif
其他品牌 :
Geneka Biotechnology
产品类型 :
抗体
产品名称 :
RNA Pol II CTD phospho Tyr1 antibody (mAb)
目录 :
61383
规格 :
100微克
价格 :
350美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
3D12
反应物种 :
人类, S. cerevisiae
应用 :
免疫印迹, 免疫沉淀, 染色质免疫沉淀
更多信息或购买 :
Active Motif 产品网页
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫沉淀; S. cerevisiae; 图 S1
Harlen K, Trotta K, Smith E, Mosaheb M, Fuchs S, Churchman L. Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue. Cell Rep. 2016;15:2147-2158 pubmed 出版商
  • 免疫印迹; 人类; 1:200; 图 2
Zaborowska J, Baumli S, Laitem C, O Reilly D, Thomas P, O Hare P, et al. Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation. PLoS ONE. 2014;9:e107654 pubmed 出版商
产品信息
目录代码 :
61383
产品名称 :
RNA Pol II CTD phospho Tyr1 antibody (mAb)
宿主物种 :
大鼠
克隆性 :
单克隆
抗原修饰 :
磷酸化
规格 :
100微克
标价 :
350美元
克隆 :
3D12
抗体亚型 :
IgG1
交叉活性 :
Human, Other (Wide Range)
背景 :
RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination.
免疫原 :
This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
用途 :
ChIP, 免疫沉淀, 免疫印迹
储存 :
Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
产品引用 :
References: The use of this antibody is described in the following Reference:ChIP, IP & WB: Mayer, A. et al. (2012) Science 336:1723-1725.
更多信息或购买 :
Active Motif 产品网页
公司信息
Active Motif
787 Kangqiao Road，Building 10, Suite 202，Pudong District，Shanghai, China
techchina@activemotif.com
http://www.activemotif.com
86-21-20926090 400-018-8123
公司总部: 美国