产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pScI_dCas9-CDA_J23119-sgRNA
目录 :
108550
更多信息或购买 :
文章摘录数: 1
参考文献
Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018;3:423-429 pubmed 出版商
产品信息
目录号 :
108550
产品名称 :
pScI_dCas9-CDA_J23119-sgRNA
文章 :
doi10.1038/s41564-017-0102-6 [pii]
id28192964
pubmed_id29403014
细菌的抗性 :
氯霉素
克隆 :
backbonepSC101
backbone_mutation
backbone_origin
backbone_size9263
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
生长株 :
Bacterial Target-AID vector with sgRNA
生长温度 :
Allow cells to recover for 2 hr in SOC medium. Transformed cells grow slow and colony formation takes 2 days. Do not pick larger colonies as escape cells may appear.
起源 :
30
主要研究者 :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterlambda OR
sequencing_primer_3M13F
sequencing_primer_5taaatctatcaccgcaaggg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
OYN72315.1
ABO15149.1
mutationD10A and H840A for SpCas9
namedCas9-PmCDA1
shRNA_sequence
size7252
species
100
Synthetic
32644
Other
Streptococcus pyogenes
tags
location
tagFlag
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterJ23119
sequencing_primer_3gcgtggatccggcttac
sequencing_primer_5ggccctgcaaccattatc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameStreptococcus pyogenes sgRNA
shRNA_sequence
size138
species
100
Synthetic
tags
抗性标记物 :
2898
标签 :
Low Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国