产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pEJS578_DD-dSpyCas9-mCherry-APEX2
目录 :
108570
更多信息或购买 :
Addgene 产品网页
文章摘录数: 2
参考文献
Briggs E, Mita P, Sun X, Ha S, Vasilyev N, Leopold Z, et al. Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9. Mob DNA. 2021;12:21 pubmed 出版商
Lee H, Lee I, Kim J, Lee S, Kwak S, Suh M, et al. Phosphorylation of OGFOD1 by Cell Cycle-Dependent Kinase 7/9 Enhances the Transcriptional Activity of RNA Polymerase II in Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed 出版商
产品信息
目录号 :
108570
产品名称 :
pEJS578_DD-dSpyCas9-mCherry-APEX2
文章 :
doi10.1038/s41592-018-0006-2
id28192847
pubmed_id
细菌的抗性 :
氨苄西林
克隆 :
backbonepHAGE-DEST
backbone_mutation
backbone_origin
backbone_size6000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
生长记录 :
CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529 537.
生长株 :
Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5MluI
promoterCMV
sequencing_primer_3
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedSpyCas9
shRNA_sequencegaatctgcaagtggatatt
size4113
species
100
Synthetic
tags
locationN terminal on insert
tagDestabilization domains
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagFlag
locationC terminal on insert
tagAPEX2
质粒拷贝数 :
pHAGE-TO-DD-dCas9-mCherry was a gift from David Grunwald lab (published in Ma et al J. Cell Biol. 2016).
抗性标记物 :
1713
标签 :
High Copy
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国