HSL2 D04_pECIA14 | Addgene 114890 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

HSL2 D04_pECIA14

114890

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Smakowska Luzan E, Mott G, Parys K, Stegmann M, Howton T, Layeghifard M, et al. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Nature. 2018;553:342-346 pubmed 出版商

产品信息

目录号 :

114890

产品名称 :

HSL2 D04_pECIA14

文章 :

doi	10.1038/nature25184
id	28196350
pubmed_id	29320478

细菌的抗性 :

氨苄西林

克隆 :

backbone

pECIA14

backbone_mutation

backbone_origin

Chris Garcia (Addgene plasmid # 47051)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

生长记录 :

SNGDAEILSRVKKTRLFDPDGNLQDWVITGDNRSPCNWT
GITCHIRKGSSLAVTTIDLSGYNISGGFPYGFCRIRTLI
NITLSQNNLNGTIDSAPLSLCSKLQNLILNQNNFSGKLP
EFSPEFRKLRVLELESNLFTGEIPQSYGRLTALQVLNLN
GNPLSGIVPAFLGYLTELTRLDLAYISFDPSPIPSTLGN
LSNLTDLRLTHSNLVGEIPDSIMNLVLLENLDLAMNSLT
GEIPESIGRLESVYQIELYDNRLSGKLPESIGNLTELRN
FDVSQNNLTGELPEKIAALQLISFNLNDNFFTGGLPDVV
ALNPNLVEFKIFNNSFTGTLPRNLGKFSEISEFDVSTNR
FSGELPPYLCYRRKLQKIITFSNQLSGEIPESYGDCHSL
NYIRMADNKLSGEVPARFWELPLTRLELANNNQLQGSIP
PSISKARHLSQLEISANNFSGVIPVKLCDLRDLRVIDLS
RNSFLGSIPSCINKLKNLERVEMQENMLDGEIPSSVSSC
TELTELNLSNNRLRGGIPPELGDLPVLNYLDLSNNQLTG
EIPAELLRLKLNQFNVSDNKLYGKIPSGFQQDIFRPSFL
GNPNLCAPNLDPIRPCRSKR
Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence:

生长株 :

Prey vector HSL2 D04_pECIA14 should be used with bait vector HSL2 D04_pECIA2.

起源 :

主要研究者 :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	Metallothionein (Copper-Inducible)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

AT5G65710

shRNA_sequence

size

species

3702

Arabidopsis thaliana