产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
CDK1as_T2A_puromycin
目录 :
118595
更多信息或购买 :
Addgene 产品网页
文章摘录数: 1
参考文献
Saldivar J, Hamperl S, Bocek M, Chung M, Bass T, Cisneros Soberanis F, et al. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018;361:806-810 pubmed 出版商
产品信息
目录号 :
118595
产品名称 :
CDK1as_T2A_puromycin
文章 :
doi10.1126/science.aap9346
id28196772
pubmed_id30139873
细菌的抗性 :
氨苄西林
克隆 :
backbonepT2/SVNeo, a gift from Perry Hackett (Addgene 26553 Cui et al., 2002)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长记录 :
Please also cite the following publication: Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68. PubMed PMID: 17635936; PubMed Central PMCID: PMC2064445.
生长株 :
One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5NruI
promoterCMV
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutation
nameCDK1
shRNA_sequence
size
species
8355
Xenopus laevis
tags
抗性标记物 :
4203
标签 :
High Copy
条款 :
Puromycin
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国