pAG222 PIK3CG 3'UTR mut | Addgene 12052 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pAG222 PIK3CG 3'UTR mut

12052

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Farh K, Grimson A, Jan C, Lewis B, Johnston W, Lim L, et al. The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science. 2005;310:1817-21 pubmed

产品信息

目录号 :

12052

产品名称 :

pAG222 PIK3CG 3'UTR mut

文章 :

doi	10.1126/science.1121158
id	717
pubmed_id	16308420

细菌的抗性 :

氨苄西林

克隆 :

backbone

pIS2

backbone_mutation

backbone_origin

pIS2 derived from pRL-SV40 (Promega)

backbone_size

3700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

生长记录 :

PIK3CG 3'UTR mutant in renilla luciferase reporter plasmid.

起源 :

主要研究者 :

alt_names

PIK3CG

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	SacI
promoter
sequencing_primer_3
sequencing_primer_5	EBV rev primer (GTGGTTTGTCCAAACTCATC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
gene	PIK3CG
id	5294

genbank_ids

NM_002649

mutation

Mutations in microRNA recognition sites (seed matches).

name

N23 3'UTR mut

shRNA_sequence

size

600

species

9606

Homo sapiens