pcDNA5/FRT/TO-GAlpha15-RLuc8 | Addgene 140984 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pcDNA5/FRT/TO-GAlpha15-RLuc8

140984

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Olsen R, Diberto J, English J, Glaudin A, Krumm B, Slocum S, et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat Chem Biol. 2020;: pubmed 出版商

产品信息

目录号 :

140984

产品名称 :

pcDNA5/FRT/TO-GAlpha15-RLuc8

文章 :

doi	10.1038/s41589-020-0535-8
id	28211044
pubmed_id	32367019

细菌的抗性 :

氨苄西林

克隆 :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5203

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

生长记录 :

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

生长株 :

Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

起源 :

主要研究者 :

alt_names

GNA15

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	TAGAAGGCACAGTCGAGG
sequencing_primer_5	CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GNA16, HG1L
gene	GNA15
id	2769

genbank_ids

mutation

RLuc8 and flanking SGGGGS linkers have been inserted at amino acid position 245 of the alpha subunit

name

GAlpha15-RLuc8

shRNA_sequence

size

2094

species

9606

Homo sapiens