产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pJRL2 GAL1pr VYFP(EB#1571)
目录 :
14847
更多信息或购买 :
文章摘录数: 1
参考文献
Raser J, O Shea E. Control of stochasticity in eukaryotic gene expression. Science. 2004;304:1811-4 pubmed
产品信息
目录号 :
14847
产品名称 :
pJRL2 GAL1pr VYFP(EB#1571)
文章 :
doi10.1126/science.1098641
id1373
pubmed_id15166317
细菌的抗性 :
氨苄西林
克隆 :
backbonepJRL2
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
生长记录 :
pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3 to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA.
起源 :
37
主要研究者 :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBR020W
geneGAL1
id852308
genbank_ids
mutation
nameGAL1 promoter
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagVYFP
抗性标记物 :
373
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国