产品简要
文章摘录数: 1
参考文献 |
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Raser J, O Shea E. Control of stochasticity in eukaryotic gene expression. Science. 2004;304:1811-4 pubmed
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产品信息
目录号 :
14847
产品名称 :
pJRL2 GAL1pr VYFP(EB#1571)
文章 :
doi | 10.1126/science.1098641 |
id | 1373 |
pubmed_id | 15166317 |
细菌的抗性 :
氨苄西林
克隆 :
backbone | pJRL2 | |
backbone_mutation | ||
backbone_origin | ||
backbone_size | ||
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
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生长记录 :
pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3 to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA.
起源 :
37
主要研究者 :
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抗性标记物 :
373 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国