产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
ZE9 tiggywinkle hedgehog pT7TS
目录 :
17058
更多信息或购买 :
产品信息
目录号 :
17058
产品名称 :
ZE9 tiggywinkle hedgehog pT7TS
文章 :
doi
id1885
pubmed_id
细菌的抗性 :
氨苄西林
克隆 :
backboneT7TS
backbone_mutation
backbone_origin
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Expression
生长记录 :
Steve Ekker clone A, in vector pT7TS. Citation: Ekker et al. (1995), Current Biology 5:944-955. T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis -globin 5 and 3 untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5 -GGACTAGTCACCATGGA-CGTAAGGCTGCA-3 ), and (5 -AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3 ).
起源 :
37
主要研究者 :
alt_names
twhh
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5SpeI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasestwh, twhh
geneshhb
id30444
genbank_ids
mutation
nametiggywinkle hedgehog
shRNA_sequence
size
species
7955
Danio rerio
tags
抗性标记物 :
348
标签 :
未知
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国