产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pFLAG-Egfr(Velvet)
目录 :
18788
更多信息或购买 :
文章摘录数: 1
参考文献
Du X, Tabeta K, Hoebe K, Liu H, Mann N, Mudd S, et al. Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Genetics. 2004;166:331-40 pubmed
产品信息
目录号 :
18788
产品名称 :
pFLAG-Egfr(Velvet)
文章 :
doi10.1534/genetics.166.1.331
id2173
pubmed_id15020428
细菌的抗性 :
氨苄西林
克隆 :
backbonepFLAG-CMV3
backbone_mutation
backbone_originSigma
backbone_size6280
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
depositor comments :
The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24
生长温度 :
37
插入 :
alt_names
Egfr
cloning
clone_methodRestriction Enzyme
cloning_site_3Bgl II
cloning_site_5Hind III
promoter
sequencing_primer_3C-CMV-24 sequencing primer
sequencing_primer_5N-CMV-30 sequencing primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliases9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2
geneEgfr
id13649
genbank_ids
mutationD833G; signal sequence removed (aa1-24)
nameepidermal growth factor receptor
shRNA_sequence
size3561
species
10090
Mus musculus
tags
locationN terminal on backbone
tagFLAG
主要研究者 :
33
质粒拷贝数 :
High Copy
抗性标记物 :
Neomycin (select with G418)
条款 :
MTA
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国