产品简要
文章摘录数: 4
参考文献 |
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产品信息
目录号 :
19123
产品名称 :
shCD44-2 pRRL
文章 :
doi | 10.1016/j.cell.2008.06.006 |
id | 2244 |
pubmed_id | 18614011 |
细菌的抗性 :
氨苄西林
克隆 :
backbone | pRRL-U6-siluc-cppt-hPGKGFPSIN | ||
backbone_mutation | |||
backbone_origin | |||
backbone_size | 7247 | ||
promoter | |||
sequencing_primer_3 | |||
sequencing_primer_5 | |||
vector_types |
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生长记录 :
Please note that the shRNA oligo present in this plasmid is referred to as shCD44-3 in the associated publication.
sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCG
AGACAATCAGTAGCACATTGCATTTTTTG-3'
shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence shRNA oligo sequence:
sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCG
AGACAATCAGTAGCACATTGCATTTTTTG-3'
shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence shRNA oligo sequence:
生长温度 :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.
起源 :
37
主要研究者 :
|
抗性标记物 :
568 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国