pBABE Gal4(1-94)HSF ABC FLAG标签pH值 | Addgene 1947 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pBABE Gal4(1-94)HSF ABC FLAG标签pH值

1947

更多信息或购买 :

Addgene 产品网页

文章摘录数: 1

参考文献
Sullivan E, Weirich C, Guyon J, Sif S, Kingston R. Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. Mol Cell Biol. 2001;21:5826-37 pubmed

产品信息

目录号 :

1947

产品名称 :

pBABE Gal4(1-94)HSF ABC FLAG标签pH值

文章 :

doi	10.1128/MCB.21.17.5826-5837.2001
id	141
pubmed_id	11486022

细菌的抗性 :

氨苄西林

克隆 :

backbone

pBABE-puro

backbone_mutation

backbone_origin

backbone_size

5200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

生长记录 :

contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Phenylalanine Mutant. (Kingston #1154)

起源 :

主要研究者 :

alt_names

HSF1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI/BglII
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	HSTF1
gene	HSF1
id	3297

genbank_ids

mutation

phenylalanine mutant (F418, F492, F500 A)

name

Heat Shock Factor 1

shRNA_sequence

size

1300

species

9606

Homo sapiens