产品简要
文章摘录数: 3
| 参考文献 |
|---|
Ong C, Cheng H, Chang L, Ohtsuka T, Kageyama R, Stormo G, et al. Target selectivity of vertebrate notch proteins. Collaboration between discrete domains and CSL-binding site architecture determines activation probability. J Biol Chem. 2006;281:5106-19 pubmed
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产品信息
目录号 :
20185
产品名称 :
3XFlagNICD3
文章 :
| doi | 10.1074/jbc.M506108200 |
| id | 2425 |
| pubmed_id | 16365048 |
细菌的抗性 :
氨苄西林
克隆 :
| backbone | p3XFLAG-CMV-7 | |
| backbone_mutation | ||
| backbone_origin | Sigma | |
| backbone_size | 4700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
生长记录 :
All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3 (5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
起源 :
37
主要研究者 :
|
质粒拷贝数 :
Notch 3 plasmid from Dr. Urban Lendahl (Karolinska Institutet, Stockholm, Sweden).
抗性标记物 :
| 1405 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国