pWZL Neo Myr Flag PIK3CG | Addgene 20574 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pWZL Neo Myr Flag PIK3CG

20574

更多信息或购买 :

Addgene 产品网页

文章摘录数: 3

参考文献
Khater M, Wei Z, Xu X, Huang W, Lokeshwar B, Lambert N, et al. G protein βγ translocation to the Golgi apparatus activates MAPK via p110γ-p101 heterodimers. J Biol Chem. 2021;:100325 pubmed 出版商
Pei Y, Hwang N, Lang F, Zhou L, Wong J, Singh R, et al. Quassinoid analogs with enhanced efficacy for treatment of hematologic malignancies target the PI3Kγ isoform. Commun Biol. 2020;3:267 pubmed 出版商
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

产品信息

目录号 :

20574

产品名称 :

pWZL Neo Myr Flag PIK3CG

文章 :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

细菌的抗性 :

氨苄西林

克隆 :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

生长记录 :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

生长温度 :

Stbl3 cells to prevent recombination.

起源 :

主要研究者 :

alt_names

phosphoinositide-3-kinase, catalytic, gamma polypeptide

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
gene	PIK3CG
id	5294

genbank_ids

NM_002649

mutation

name

PIK3CG

shRNA_sequence

size

species

9606

Homo sapiens