产品简要
文章摘录数: 1
| 参考文献 |
|---|
Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark S, et al. A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response. J Cell Biol. 2002;158:639-46 pubmed
|
产品信息
目录号 :
21824
产品名称 :
SREC 1: abu-1::gfp
文章 :
| doi | 10.1083/jcb.200203086 |
| id | 2769 |
| pubmed_id | 12186849 |
细菌的抗性 :
氨苄西林
克隆 :
| backbone | pPD95_75 | |
| backbone_mutation | ||
| backbone_origin | Available at Addgene (#1494) | |
| backbone_size | 4487 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
生长记录 :
Addgene sequencing results show several mismatches with author's sequence. abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.
起源 :
37
主要研究者 :
|
质粒拷贝数 :
pPD95.75 was a gift from Dr. Andy Fire
抗性标记物 :
| 421 |
标签 :
未知
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国