产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pCMV4 p65
目录 :
21966
更多信息或购买 :
文章摘录数: 35
参考文献
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Shen Y, Kapfhamer D, Minnella A, Kim J, Won S, Chen Y, et al. Bioenergetic state regulates innate inflammatory responses through the transcriptional co-repressor CtBP. Nat Commun. 2017;8:624 pubmed 出版商
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Xiang S, Zhang Q, Tang Q, Zheng F, Wu J, Yang L, et al. Activation of AMPK? mediates additive effects of solamargine and metformin on suppressing MUC1 expression in castration-resistant prostate cancer cells. Sci Rep. 2016;6:36721 pubmed 出版商
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Li L, Wu J, Zheng F, Tang Q, Wu W, Hann S. Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I. J Exp Clin Cancer Res. 2016;35:112 pubmed 出版商
Cui X, Kong C, Zhu Y, Zeng Y, Zhang Z, Liu X, et al. miR-130b, an onco-miRNA in bladder cancer, is directly regulated by NF-?B and sustains NF-?B activation by decreasing Cylindromatosis expression. Oncotarget. 2016;7:48547-48561 pubmed 出版商
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Harris D, Chandrasekharan U, Bandyopadhyay S, Willard B, DiCorleto P. PRMT5-Mediated Methylation of NF-κB p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-α and IFN-γ Costimulation. PLoS ONE. 2016;11:e0148905 pubmed 出版商
Balasubramaniyan N, Ananthanarayanan M, Suchy F. Nuclear factor-?B regulates the expression of multiple genes encoding liver transport proteins. Am J Physiol Gastrointest Liver Physiol. 2016;310:G618-28 pubmed 出版商
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产品信息
目录号 :
21966
产品名称 :
pCMV4 p65
文章 :
doi10.1073/pnas.89.5.1875
id2813
pubmed_id1542686
细菌的抗性 :
氨苄西林
克隆 :
backbonepCMV4
backbone_mutation
backbone_originAndersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
backbone_size4874
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
起源 :
37
主要研究者 :
alt_names
RelA
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5CMV forward: cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIF3BL3, CMCU, NFKB3, p65
geneRELA
id5970
genbank_ids
mutationThis construct was the product of 3 subcloning procedures: 1. XbaI-EcoRI-p65-EcoRI-KpnI from pBluescript II SK +/- was digested with XbaI/KpnI, and subcloned to M13 mp19. 2. HindIII-XbaI-p65-HindIII-KpnI from the M13 mp19 construct was digested with HindIII, then subcloned into pCMV4. 3. The resulting pCMV4 p65 plasmid can be liberated using HindIII, with a size of ~2.5 kb. The large size (p65 is only 1.5kb) is due to the inclusion of multiple polylinkers from pBluescript and M13mp19 as well as non-coding regions of p65.
nameNFkB p65 subunit
shRNA_sequence
size2500
species
9606
Homo sapiens
tags
抗性标记物 :
176
标签 :
Low Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国