产品简要
公司名称 :
Addgene
产品类型 :
cDNA
产品名称 :
pcDNA3质粒FLAG标签
目录 :
22893
更多信息或购买 :
Addgene 产品网页
文章摘录数: 2
参考文献
Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017;66:622-634.e8 pubmed 出版商
Unsal Kaçmaz K, Chastain P, Qu P, Minoo P, Cordeiro Stone M, Sancar A, et al. The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Mol Cell Biol. 2007;27:3131-42 pubmed
产品信息
目录号 :
22893
产品名称 :
pcDNA3质粒FLAG标签
文章 :
doi10.1128/MCB.02190-06
id3035
pubmed_id17296725
细菌的抗性 :
氨苄西林
克隆 :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
生长记录 :
There is one point mutation in the insert, but the protein expressed with this construct forms complexes with the other subunits of the RPA heterotrimer, binds other known interacting proteins (XPA, Tipin), and shows an enrichment in the chromatin fraction of cells treated with replication stress-inducing agents. So by these criteria the construct appears to be WT.
起源 :
37
主要研究者 :
alt_names
RPA34
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesREPA2, RP-A p32, RP-A p34, RPA32
geneRPA2
id6118
genbank_ids
mutation
nameRPA2
shRNA_sequence
size810
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
抗性标记物 :
436
标签 :
High Copy
条款 :
Neomycin (select with G418)
更多信息或购买 :
Addgene 产品网页
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
公司总部: 美国