pDONR223-MAPK1 | Addgene 23498 产品信息

产品简要

公司名称 :

Addgene

产品类型 :

cDNA

产品名称 :

pDONR223-MAPK1

23498

更多信息或购买 :

Addgene 产品网页

文章摘录数: 5

参考文献
Sun H, Zhu A, Gao Y, Terajima H, Fei Q, Liu S, et al. Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. Mol Cell. 2020;80:633-647.e7 pubmed 出版商
Satoh T, Mellett M, Meier Schiesser B, Fenini G, Otsuka A, Beer H, et al. IL-36γ drives skin toxicity induced by EGFR/MEK inhibition and commensal Cutibacterium acnes. J Clin Invest. 2019;: pubmed 出版商
Wu J, Xiang S, Zhang M, Fang B, Huang H, Kwon O, et al. Histone deacetylase 6 (HDAC6) deacetylates extracellular signal-regulated kinase 1 (ERK1) and thereby stimulates ERK1 activity. J Biol Chem. 2018;293:1976-1993 pubmed 出版商
Turski M, Brady D, Kim H, Kim B, Nose Y, Counter C, et al. A novel role for copper in Ras/mitogen-activated protein kinase signaling. Mol Cell Biol. 2012;32:1284-95 pubmed 出版商
Johannessen C, Boehm J, Kim S, Thomas S, Wardwell L, Johnson L, et al. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 2010;468:968-72 pubmed 出版商

产品信息

目录号 :

23498

产品名称 :

pDONR223-MAPK1

文章 :

doi	10.1038/nature09627
id	3878
pubmed_id	21107320

细菌的抗性 :

大观霉素

克隆 :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

生长记录 :

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

起源 :

主要研究者 :

alt_names

mitogen-activated protein kinase 1

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK
gene	MAPK1
id	5594

genbank_ids

mutation

missing first codon

name

MAPK1

shRNA_sequence

size

1085

species

9606

Homo sapiens